Glycoproteins Analysis Based on HPLC
A sample combination or analyte is pumped down a column containing chromatographic packing material (stationary phase) at high pressure using a solvent known as the mobile phase in high-performance liquid chromatography (HPLC). A stream of flowing nitrogen or helium carrier gas carries the sample. Any substance that can dissolve in a liquid and have trace amounts as low as parts per trillion can be separated and identified using HPLC. The primary benefits of HPLC are its high productivity in terms of cost parameters, great resolution, ease of use, generally high recoveries, and excellent reproducibility of repeat separations.
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